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Graph algorithms for calculating the distribution of the Amur tiger tracks in Primorsky Krai |
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G.Sh. Tsitsiashvili, V.N. Bocharnikov, S.M. Krasnopeev, M.A. Osipova |
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Section: Methodology and research methods. Models and forecasts
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The paper analyzes the distribution of the Amur tiger (Panthera tigris altaica) tracks in the Primorsky Krai municipalities according to observations obtained during the one-time winter count organized by the Pacific Institute of Geography of the Far Eastern Branch of the Russian Academy of Sciences in 2005. This task is related to the need to calculate the number of animals based on the tracks of their vital activity. Based on the initial data we form a sample to analyze a set of tracks to identify intersection areas of various tiger habitats in Primorsky Krai. The map of Primorsky Krai municipalities was presented in the form of a planar graph to identify their intersections. A recurrent algorithm for identifying tiger clusters was developed using graph theory methods. According to them, the territory of Primorsky Krai is divided into northern, central, southern and southwestern zones with intersections. The districts of Primorsky Krai included in the designated zones are listed. The total number of tracks in intersecting adjacent zones was counted to identify possible tiger migrations in Primorsky Krai. A quantitative assessment of the degree of intersection of the selected zones by the number of tiger tracks is given. The proposed calculation scheme can be used to clarify and verify the results of modeling the spatial distribution and total population size of the Amur tiger. |
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Keywords: tiger clusters, planar graphs, number of tracks, zones of influence
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Article published in number 3 for 2024
DOI: 10.25750/1995-4301-2024-3-020-028 |
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Abiotic factors affect the Phragmites australis productivity in lakes in the south of Western Siberia |
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E.Yu. Zarubina |
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Section: Population ecology
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The article presents the results of studying phytomass, stem height and density of thickets (herbage) of Phragmites australis, one of the most common aerial-aquatic plant in the south of Western Siberia. This region is distinguished by a rare combination of chloride, sulfate and soda lakes in a relatively small space. As an eurybiont, reed is highly resistant to water and soil salinity, but high salinity adversely affects its growth and development. The aim of the work was to assess the degree of abiotic factors (pH, mineralization) effect on the quantitative characteristics of reed growing in water bodies with different water mineralization: from 0.005 to 387.6 g/L. In total, we studied 59 freshwater, brackish-water (oligo-, meso- and polyhaline) and hypersaline water bodies, located in various natural zones (southern taiga, forest,
forest-steppe, steppe and dry-steppe) in the south of Western Siberia. The studies were carried out in late July – early August from 2012 to 2020 during the period of maximum vegetation of plants. As a result of the studies, it is shown that the optimal for reed growth and development is water mineralization from 0.5 to 5.0 g/L, when the highest values of biomass and plant height are observed. A model based on redundancy analysis (RDA) was built and a correlation analysis was carried out to assess the dependence of phytomass, shoot height and herbage density on abiotic factors. We took into account pH, salinity, and Ca2+, Na+, Mg2+, K+, HCO3-, Cl- and SO42- content. We found that pH and determined by Ca2+ water hardness are the limiting factors in freshwater lakes. In brackish-water meso- and polyhaline, as well as in saline hyperhaline water bodies reed phytomass is affected by a whole range of factors, while shoot height is affected by pH, chlorides, and water hardness, and grass stand density is affected by chlorides and sulfates. |
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Keywords: Phragmites australis, phytomass, shoot height, density, salinity, pH
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Article published in number 3 for 2024
DOI: 10.25750/1995-4301-2024-3-200-209 |
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Individual and geographic variability of Picea obovata cones in the Russian part of its area in relation to the seeds quantity in them |
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P.P. Popov, M.N. Kazantseva, S.P. Arefyev |
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Section: Population ecology
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The cone length of Picea obovata Ledeb. is closely related to the seeds quantity that causes the importance of full-scale study of this indicator. Studies were carried out in the entire Russian part of the P. obovata range from Murmansk region to Transbaikalia and Yakutia. It was found that geographical variability of cone length is relatively small (44–77 mm) and significantly inferior to individual variability (45–110 mm). The coefficient of a variation is 10 and 14%, respectively. We found that in the total population of Picea abies (L.) H. Karst. and P. obovata determined by their strong introgressive hybridization, cones of P. obovata is the category small and average. It is reasonable to distinguish an additional category of very small cones. The established categories of cones are characterized by the following length: very small 50 (54 mm and less), small 60 (55–64 mm) and medium 70 (65 mm and more). The approximate number of full seeds in them at good yields is 76 (less in extreme conditions of the Far North), 78–92 and 94–108 pieces, respectively. These figures are important for assessing the processes of spruce forest regeneration. Geographical distribution of average cone length in the studied territory it is not clearly expressed. The smallest cones are characteristic of spruce populations along Arctic Circle and in the Polar Regions, while in populations of the southern part of the range they are much larger. The majority of the Russian populations of P. obovata (about 2/3) have cones 65–70 mm long. The content of full seeds in cones is rather small. |
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Keywords: Picea obovata, species range, Russia, cone length, number of seeds in cones
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Article published in number 3 for 2024
DOI: 10.25750/1995-4301-2024-3-192-199 |
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The potential of plastic biodegradation by basidial fungi and actinobacteria (review) |
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I.G. Shirokikh, А.А. Shirokikh |
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Section: Theoretical problems of ecology
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With the ever-increasing production and consumption of plastics, inadequate management of plastic waste and its improper disposal pose a significant threat to the environment, affecting all forms of life, natural ecosystems and economies worldwide. In the face of this threat the search for alternative environmentally friendly solutions such as biodegradation instead of traditional recycling is of paramount importance. Currently knowledge about the mechanisms and effectiveness of plastics biodegradation is focused mainly on such groups of microorganisms as Gram-negative bacteria and fungi ascomycetes. The aim of this review is to highlight the ideas available in the literature about the potential decomposition of the most common plastic waste (polyethylene, polypropylene, polyvinyl chloride, polyethylene terephthalate and polyurethane) as a result of biotic processes. Basidial fungi and actinobacteria have not yet found practical application
in the technologies of processing and utilization of synthetic plastics. We discuss the ability of specific representatives of the two above taxonomic groups to decompose various synthetic polymers. The unique strategies of basidial fungi such as a powerful enzymatic system, the ability to absorb and produce natural biosurfactants – hydrophobins, allowed fungi to use plastics as a source of carbon and energy are considered. The possible contribution of mainly thermophilic
actinobacteria of the genera Thermobifida, Thermoactinomyces, Thermomonospora, Saccharomonospora, Actinomadura, Microbispora and Streptomyces in improving the plastic waste disposal in order to create effective and sustainable plastic management practices has been characterized. |
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Keywords: pollution, plastic waste, biodegradation, microorganisms, enzymatic systems
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Article published in number 2 for 2024
DOI: 10.25750/1995-4301-2024-2-006-021 |
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Obtaining heterological rhamnolipid producers for industrial production of highly-effective biosurfactant |
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M.N. Baramzin, S.G. Litvinets, E.A. Martinson |
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Section: Remediation and rehabilitation
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Rhamnolipids are promising microbial glycolipid biosurfactants that can be used as preparations for soil bioremediation in oil pollution. The expression of the rhamnolipid biosynthesis gene cluster in the main producer, Pseudomonas aeruginosa, is regulated by quorum sensing signals (QS). Complex biosynthesis regulation does not allow achieving high product yields. The production of heterologous rhamnolipid producers is a promising way to overcome the complex QS-dependent regulation of rhamnolipid biosynthesis, and also avoids the use of opportunistic Pseudomonas aeruginosa for the industrial production of rhamnolipids. Heterologous Escherichia coli BLWT-based producers with a transformant E14 productivity of 3.40±0.96 g/L were obtained on the basis of the plasmid vector pAl2–T with a construct from the native promoter and rhlAB genes, and Pseudomonas viridiflava AlC1223-based ones with the productivity of transformants P5, P6, P8 332.00±0.10, 200.00±0.10 and 180.00±0.10 g/L, respectively. The presence of rhlAB genes in the expression vector allows the obtaining monorhamnolipids for further use as an agent for the solubilization of soil hydrophobic pollutant (e. g. petroleum products), which will intensify bioremediation processes. |
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Keywords: rhamnolipids, heterologous expression, pseudomonads, biosurfactants, oil degraders, glycolipids
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Article published in number 2 for 2024
DOI: 10.25750/1995-4301-2024-2-135-142 |
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Biological contamination of soils by livestock by-products |
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N.V. Syrchina, L.V. Pilip, E.P. Kolevatykh, T.Ya. Ashikhmina |
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Section: Ecology and climate change
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By-products of animal husbandry (BPAH) such as manure, manure effluents (ME) are widely used as organic
fertilizers. The main disadvantage of the appropriate fertilizers is the high content of microorganisms (MO) untypical to uncontaminated natural environments, including pathogenic ones. Currently used natural methods of BPAH neutralization can significantly reduce the risks of transferring pathogenic and opportunistic MO into fertilized soil, but do not completely exclude them. The study examined the effects of long-term (more than 7 years) introduction of a neutralized liquid fraction (LF) of pork ME and cattle ME on the chemical composition and sanitary and hygienic condition of the soil of arable fields and wooded areas located at 500 m from the boundaries of the fields. Prolonged application of LF led to an increase in the content of organic matter, mobile phosphorus, potassium and sulfur, an increase in the amount of absorbed bases, a decrease in metabolic acidity in arable land compared with adjacent forest areas. However the sanitary condition of both arable land and adjacent territories deteriorated significantly. A significant increase in the total microbial number (TMN) was revealed in all soil samples in comparison to the normative indicators. Particularly high values of TMN were found in soil fertilized with cattle manure. The corresponding soil was characterized by the highest
content of organic matter and neutral reactions of the environment, which provided favorable conditions for the active development of saprophytic microbiota. Fresh faecal contamination was detected in all selected soil samples. Soils are characterized as highly polluted. According to the indicators of the index Escherichia coli, Enterococcus and Clostridium perfringens, the soils of all studied sites are moderately dangerous. Higher values of the corresponding indices were found in arable land fertilized by the LF of pork farms and in the territories adjacent to arable land. In the course of sanitary and bacteriological studies, eggs of helminthes were not found in the soil. |
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Keywords: by-products of animal husbandry, organic fertilizers, soil sanitation, biological pollution
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Article published in number 2 for 2024
DOI: 10.25750/1995-4301-2024-2-201-210 |
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Some patterns of the accumulation of trace elements in the leaves of linden (Tilia cordata Mill.) and birch (Betula alba L.) |
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A.V. Gorbunov, D.B. Petrenko, A.A. Dronova |
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Section: Chemistry of natural environments and objects
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The purpose of the work: to study the patterns of background accumulation of trace elements during the spring-summer-autumn season in the vegetative organs (leaves) of the heart-shaped linden (Tilia cordata Mill.) and white birch (Betula alba L.). Samples were taken on the territory of the Main Botanical Garden of the Russian Academy of Sciences during one season of 2018 from the same tree with an interval of 10–12 days, starting from the opening of the bud (end of April) right up to the time of leaf fall. Soil samples were also taken from birch and linden growing areas. The samples were analyzed in the laboratory of Chemical analytical methods of the GIN RAS using X-ray fluorescence analysis (XRF), instrumental neutron activation analysis (INАA) and atomic absorption spectrometry of cold vapor (AA). The concentration of trace elements in the selected soil samples corresponds to the concept of “background” contents. Two types of trace elements’ distribution in linden and birch leaves during the season have been revealed. The first type is a uniform accumulation of trace elements with a maximum at the end of the season (Na, Ca, Cr, Fe, Co, Br, Sb, La). The second type of distribution is characterized by a maximum concentration of trace elements in the middle of the season with a decrease in concentration by the time of leaf fall (K, As, Zn, Cd, Pb). It should be noted that the range of changes in the concentration of trace elements during the season in both cases can reach 10–15 times. We recommend to take samples of vegetation (leaves) as soon as possible in the middle of the summer season, and to use samples taken at the same time in the same area as “background”. |
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Keywords: trace elements, accumulation, vegetative organs, higher plants
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Article published in number 2 for 2024
DOI: 10.25750/1995-4301-2024-2-063-071 |
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Study of patterns of changes in salt compositiondrill cuttings in the process of their disposal |
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E.V. Gaevaya, L.V. Rudakova |
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Section: Remediation and rehabilitation
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Drill cuttings in their natural state have viscous-plastic properties; in a dry state they are cohesive and hard. This is due to the content of sodium ions in the absorbing complex. Features of the physical and mechanical properties of drill cuttings indicate its ability to absorb and retain dissolved salts. When gypsum is added to drill cuttings in different percentages of the waste volume, the concentration of chloride ion changes in all samples, and chloride-sulfate salinization is observed. The content of sulfate ion increases with a minimum volume fraction of gypsum application. The addition of gypsum in a
volume fraction of 15 and 20% affects the reduction of phosphate and carbonate ions. A change in electrical conductivity indicates an increase in the concentration of water-soluble salts in the water extract, which is confirmed by a direct high correlation. When adding gypsum from 7–20% volume fractions, a significant reduction in the dense residue occurs. The use of gypsum increases the filtration capacity of drill cuttings. The use of gypsum makes it possible to lighten the granulometric composition of drill cuttings due to the active coagulation of colloids. As a result of the removal of water-soluble
salts, optimal water-physical properties of drill cuttings are created, structuring and filtration properties of drill cuttings are increased. A mathematical model for predicting the dissolution of salts in drill cuttings allows taking into account uncontrollable factors (density, particle size distribution, filtration coefficient, content of water-soluble salts). |
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Keywords: drill cuttings, salt composition, dissolution rate coefficient
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Article published in number 2 for 2024
DOI: 10.25750/1995-4301-2024-2-125-134 |
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The effect of radionuclide-contaminated environment on children’s heart rate |
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O.V. Kalabin, F.B. Litvin, T.M. Bruk, I.V. Bykova, E.V. Udovenko |
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Section: Social ecology
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The main aim was to study the characteristics of heart rate variability in 8- and 10-year boys and girls that permanently reside in areas with an increased level of radioactive contamination. The body functions are formed by close interaction of the body and the environment. The adaptive responses of the developing organism are improved at each ontogenetic stage as its contact with the environment become more complex. We found that features of heart rate regulation depend on the area of residence. The contribution of the autonomous circuit of heart rate regulation is increased for 8- and 10-year-old girls from radio-ecologically safe areas (RESA). Based on the principle of functional antagonism this leads to a weakening of the central circuit of regulation. The shift gradient of indicators is higher for 8-year-old girls from radio-ecologically unfavorable areas (REUA) compared to 10-year-old girls. This reflects the greater sensitivity of their organism to adverse factors. The dominance of the autonomous circuit of regulation was marked for 8- and 10-year-old boys from the RESA. In contrast an imbalance in the operation of regulatory mechanisms with an increase in the central regulatory circuit was found for boys from the REUA. High centralization of cardiac control indicates that adaptation mechanisms are in a state of pronounced tension. The increase in the shift gradient for boys from REUA falls at the age of 10 years. The results of the study revealed gender differences in the regulation of heart rhythm. The obtained data is important diagnostic aspects of morphofunctional maturation of the organism for subsequent ontogenetic stages depending on environmental living conditions. |
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Keywords: age, sex, radiation, heart rate variability, radio-ecological living conditions
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Article published in number 2 for 2024
DOI: 10.25750/1995-4301-2024-2-219-225 |
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Photosynthetic activity of coniferous undergrowth after industrial felling |
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V.V. Tuzhilkina |
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Section: Methodology and research methods. Models and forecasts
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The functioning of coniferous undergrowth of preliminary and subsequent generations after industrial felling was considered in the middle taiga subzone of the Komi Republic. Using spectrophotometric and gasometric methods we analyzed the pigment activity, photosynthetic, and respiratory abilities of the assimilation apparatus of pine (Pinus sylvestris L.) and spruce (Picea obovata Ledeb.) undergrowth at the 3- and 10-year old clearings. The pigment apparatus functional rearrangement of the preliminary generation spruce undergrowth at clearings was found. Needles adapt to environmental conditions at the pigment system level by changing the content and ratio of chlorophylls and carotenoids. Changes in plastids in the spruce undergrowth at the 3-year clearings occurred due to a decrease in chlorophyll and carotenoid concentrations, and at the 10-year clearings due to activation of pigment synthesis which affects the carbon dioxide absorption in chloroplasts. The assimilation apparatus of spruce undergrowth at clearing absorbs CO2 by 1.4 and 1.8 time more actively than that under the forest canopy. The photosynthetic and respiratory abilities of spruce needles at clearings do not differ. This indicates a stable level of important vital processes for the studied undergrowth at clearings during ten years after felling. Pine and spruce undergrowth needles are able to absorb CO2 at a fairly high rate up to 5.73 and 2.7 μmol CO2/(m2 · s) respectively. This rate contributes to the accumulation of assimilates in pine needles and their use for growth which logically increases the productivity of undergrowth at clearings. In general, the functional organization of spruce and pine undergrowth assimilating organs ensures their growth and favors the successful restoration of conifers at clearings in the middle taiga subzone of the European North-East. |
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Keywords: spruce, pine, undergrowth, needles, industrial felling, clearing, pigments, photosynthesis, respiration
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Article published in number 2 for 2024
DOI: 10.25750/1995-4301-2024-2-022-028 |
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ISSN 1995-4301
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