Technogenic halogenesis of oil-contaminated soils of floodplain ecosystems under conditions of humid soil formation and its environmental consequences
M.V. Nosova, V.P. Seredina
Section: Monitoring of natural and anthropogenically disturbed areas
The content and patterns of spatial distribution of readily soluble salts of technogenic origin in the soils of oil-contaminated
floodplain ecosystems of Western Siberia within the middle taiga subzone (Tomsk region) are considered. Peculiarities of
technogenic salinization – halogenesis processes that arise as a result of the operation of oilfield technological facilities (field
pipelines), are not typical for humid soil formation conditions and consist in the appearance of specific compounds of toxic salts
and signs of soil salinization. It is revealed that salinization leads to deterioration of soil fertility and the creation of adverse
conditions for the growth and development of plants. In the studied soils, technogenic salts are represented by compounds
of the toxic salts NaCl, Na2
SO4
, MgCl2
. The maximum amount of salts is recorded in the upper horizons of soils. Soils have
medium salinization (at the epicenter), and weak salinization (impact zone, pollution boundary) with a sulfate type of salinity
according to cationic composition, sodium type – according to anionic. The detected changes make it possible to assess the
ecological state of oil-contaminated soils in areas prone to excessive moisture (Western Siberia) as unsatisfactory, and to develop
a set of scientific recommendations for the implementation of the biological stage of remediation of floodplain soils. One of the
scientifically based methods may be the use of rolled biomats at the final stage of biological soil remediation. This material is
able to retain seeds of oil-resistant herbs, which are usually washed out by the rain from the soil, by creating a plant layer that
is mature in density and area, and also isolates plants from the negative effects of toxic salts formed as a result of pollution.
Article published in number 3 for 2021 DOI: 10.25750/1995-4301-2021-3-074-079
Views: 13
Dynamics of intestinal helminth fauna Perca fluviatilis (Actinopterygii: Perciformes) in conditions of long-term ecosystem succession
M.V. Rubanova, O.V. Mukhortova
Section: Theoretical problems of ecology
Modern methods of research on reservoir ecosystems are based on integrated data on the structure of communities and populations of hydrobionts. An effective way of studying changes in the structure and functioning of aquatic
ecosystems is to analyze the collections of fish parasites. The work presents the results of studies of the dynamics of the
fauna of intestinal helminths Perca fluviatilis and its relationships with some fodder objects (zooplankton, alien fish
species) in the Saratov reservoir. The possibility of practical use of helminths of predatory fish occupying the upper
level of trophic chains as “biological markers” is shown, which allow determining the degree of participation of invader
species in trophic chains and successional changes in biocenosis. Based on a comprehensive analysis of parasitological
and hydrobiological data, the trends and specifics of the 30-year period of succession of the Saratov reservoir ecosystem
associated with the spread of alien species were determined. This process includes phased introduction, naturalization, an
increase in the number of invader species, the activation of their participation in the trophic chains of hydrobionts, and a
change in the ecology of fish and parasites. An increase in the number of individual alien species and related changes in
the ecology of host species, structural and functional restructuring of populations and communities (fish, zooplankton,
zoobenthos, parasites) are predicted.
Keywords: parasites on perch, crustacean, water ecosystem transformation, reservoir, biological markers, alien species
Article published in number 3 for 2021 DOI: 10.25750/1995-4301-2021-3-031-037
Views: 3
Seed productivity and features of biology of Malaxis monophyllos (L.) Sw. (Orchidaceae) on the northern distribution border
I.A. Kirillova, D.V. Kirillov
Section: Population ecology
The article presents the results of studying some aspects of Malaxis monophyllos (L.) Sw. (Orchidaceae) reproductive biology in the Komi Republic (northeast Russia), where the northern border of this species distribution area
passes. The research was carried out in 2010–2019 in the Syktyvdinskiy and Ust’-Kulomskiy administrative districts
of the Komi Republic. We have found that M. monophyllos usually occurs in wet forests and swamps, and sometimes
occupied the disturbed habitats with low competition (old forest motorways). It usually forms small coenopopulations
(up to 100 plants), with a high proportion of generative shoots. Within the coenopopulations, individual plants form
small groups or grow as single plants. The efficiency of pollination (46%) in the study area corresponds to the average
fruit set in the whole species distribution area. This parameter varies highly within one community, depending on
the weather and climatic conditions in different years. We found statistically significant negative correlation between
fruit setting and the number of seeds in fruit. So, the lower fruit set is compensated by numerous seeds in the fruit.
Seed productivity is high. In the Komi Republic, one fruit of M. monophyllos contains an average of 3338±219 seeds,
with more than 94% of seeds with embryo. The mean index of conditionally-real seed productivity (i. e. the mean
number of seeds per a generative shoot) is 53000 psc. In the Komi Republic, the seed productivity of M. monophyllos
was found to be higher than in the southern parts of its distribution area. It can be assumed that, in the northern part
of M. monophyllos distribution area, the increased number of seeds in the fruit is one of additional mechanisms of the
implementation of seed renewal, which is the main way of reproduction for this species. The seeds of M. monophyllos
are very small – 0.33–0.09 mm, and contain 88.4% empty air space, which provides them with volatility and increases
the chance to reach suitable places for germination.
Keywords: Malaxis monophyllos (L.) Sw., Orchidaceae, the Komi Republic, northern distribution border, fruit set, seed productivity
Article published in number 3 for 2021 DOI: 10.25750/1995-4301-2021-3-133-139
Views: 7
Influence of air pollution on the ecophysiological parameters of Scots pine (Pinus sylvestris L.) and Siberian spruce Picea obovata Ledeb.) in the urban ecosystems of Southern Siberia
N.V. Pakharkova, A.D. Shkaruba, G.A. Sorokina, N.A. Gaevskiy, Yu.S. Grigoriev, O.P. Kalyakina, A.A. Shubin, M.A. Polosukhina
Section: Monitoring of natural and anthropogenically disturbed areas
This research aims towards a better understanding of the responses of the Scots pine (Pinus sylvestris L.) and the
Siberian spruce (Picea obovata Ledeb.) to air pollution stress in urban and semi-urban conditions. One of the most reliable
tests for the depth of winter dormancy is measuring the concentration of abscisic acid in needles. An alternative method
for determining the depth of winter dormancy is based on the measurements of heat-induced changes in chlorophyll fluorescence. Our research confirms that the depth of winter dormancy of P. sylvestris and P. obovata is highly sensitive
to air pollution stress. In polluted environments, in late winter and early spring, the final release from dormancy is controlled only by low temperatures and it can be easily triggered by the first warm period or even a quick daytime thawing.
Our data demonstrate that the trees growing in industrial areas are easier to release from dormancy and to be affected
by late winter or spring frost. In urban environments, the risk of frost injuries is even higher due to early spring warm
spells associated with the heat island effect. P. sylvestris is less susceptible to air pollution and temperature fluctuations
than P. obovata, and therefore represents a better choice for urban forestry projects.
Article published in number 3 for 2021 DOI: 10.25750/1995-4301-2021-3-090-097
Views: 8
Effect of the cultivation medium composition on Dunaliella tertiolecta sensitivity to potassium dichromate
E.S. Stravinskene, Yu.S. Grigor’ev, T.L. Shashkova, M.A. Subbotin, M.A. Polosukhina
Section: Ecotoxicology
The effect of a model toxicant on the growth of the algae Dunaliella tertiolecta in Goldberg medium, prepared on the
basis of solutions of various sea salt complexes, as well as NaCl alone solution, has been studied. The growth of algae in
the controls was 10–15 times after 48 hours in all variants of media. Potassium dichromate at a concentration of 5 mg/L
had little or no effect on the growth of the test culture in media prepared from the sea salt solutions. Simultaneously, the
same concentration of potassium dichromate completely suppressed the algae growth in NaCl-media. The results of the
chemical analysis of all the studied sea salt solutions showed a ratio of the main ions close to that of natural sea water. All
studied samples of sea salt solutions contained 5 to 8% magnesium and calcium ions, which is a possible explanation for
the lower sensitivity of Dunaliella tertiolecta to potassium dichromate in these media, since the hardness of the medium
affects the bioavailability of toxicants for test organisms.
Article published in number 3 for 2021 DOI: 10.25750/1995-4301-2021-3-186-192
Views: 2
Evaluation of anthropogenic load on water bodies within the city limits
T.V. Noskova, M.S. Panina, O.M. Labuzova, T.S. Papina, E.G. Ilina
Section: Monitoring of natural and anthropogenically disturbed areas
The fluorimetric analysis of the content of formaldehyde, volatile phenols and oil has been performed in the samples of
atmospheric precipitation. Atmospheric precipitation (rain and snow) were collected in Barnaul from October 2013 until September 2018. The average concentration of oil in atmospheric precipitation was 0.07 ppm, volatile phenols – 0.006 ppm and
formaldehyde – 0.1 ppm. A seasonal increase of the oil concentration was revealed in the cold period of the year. For formaldehyde
a seasonal increase of the concentration has been revealed in the warm period of the year. Volatile phenols are characterized by
a constant emission into the atmosphere of the Barnaul regardless of the time of year. The anthropogenic load of the formaldehyde, volatile phenols and oil on surface waters within the boundaries of the city of Barnaul was calculated taking into account
average rivers’ discharge. Full-flowing the Ob River does not experience significant anthropogenic load within the boundaries
of the city of Barnaul, as regards of the studied pollutants. Its waters are able to dilute the concentration of incoming studied
organic pollutants from urban stormwater runoff to before MAC. For the Barnaulka River, the stormwater runoff within the
boundaries of the city of Barnaul is a serious source of pollution with oil and volatile phenols. Phenolic compounds pose especially high risk of pollution of the Barnaulka River. Contamination of the Barnaulka River with formaldehyde is not observed.
For the improvement current unfavorable environmental situation, it will be necessary to construct the urban treatment plants.
Article published in number 3 for 2021 DOI: 10.25750/1995-4301-2021-3-098-103
Views: 20
Infestation of pea seeds (Pisum sativum L.) with pathogenic microorganisms
T.P. Gradoboeva
Section: Agroecology
Injuriousness of seed infection is very high. So, the aim of our study was estimation of pathogenic microflora on
peas seeds and screening of cultivars on seed defeat with most harmful pathogenes. As a result of perennial monitoring of
contamination of peas seeds, it is revealed annual existing of fungi of genera Ascochyta, Fusarium, Alternaria, Aspergillus,
Pennicillum, Cladosporiumas well as agents of bacteriosis Pseudomonas syringae var. pisi on their surface. Fungi of genus
Ascochyta spp. were represented with two species: A. pisi, A. pinodes. Within fungi of genus Fusarium determined on peas
seeds, F. oxysporum var. pisi, F. avenaceum, F. sporotrichioides, F. culmorum prevail. Fungi composition and defeat of
cultivars with them varied in dependence of year conditions and cultivar. Significant part in complex of seed infection for
all year of study belongs to fungi of genus Alternaria. They were met practically on seeds of all cultivars (96.5–100.0%),
with except of cultivar L-1325 (Sweden). Most dangerous present agents of diseases often exist in mycobiota of seeds:
ascochyta blight (17.4–93.5%), fusariosis (6.5–68.8%) and bacteriosis (39.1–80.0%). 237 cultivars and genotypes of
peas of domestic and foreign breeding were studied. For study period (2014–2018) seeds were defeated in the highest
degree with ascochyta blight. Hard and very hard degree of defeat of seed with this disease was noted in 27.1 and 19.8%
of genotypes. For fusariosis defeat these parameters were 2.5 and 17.3%, for bacteriosis – 31.2 and 1.4%. Absence of
ascochyta blight on seeds noted in 12.2%, fusariosis – in 31.2%, bacteriosis – 13.5% of studied cultivars. As a result of
perennial evaluation perspective genotypes were selected (Tigra (Germany), Е-3907, Е-3335, Е-3767, Е-4030, Д-23417
(Russian Federation, Kemchug (Pamir)), which had defeat of seeds with ascochyta blight, fusariosis, and bacteriosis not
higher than 4% for all years of study. This parameter has high importance in practical breeding.
Results of the phenological observations of 21 species of the Clematis L. genus relating to 4 sections and 7 subsections, which are carried out in the South-Ural botanical garden institute of UFRC RAS (Ufa) from 2007 to 2020 are presented in article. Was noted the timing of the onset of 9 phenophases: breaking of buds, beginning and end of growth of shoot, beginning, termination and duration of blossoming, maturing of fruits, termination and duration of vegetation. Correlation analysis was used to assess the relationship between phenophases, the influence of species and annual meteorological features was estimated by two-factor dispersion analysis. It was revealed that the vegetation in clematis begins mainly in the III decade of April. The phases of the beginning and end of flowering are an individual feature for each species. Fruiting usually occurs 2-2,5 months after the beginning of flowering. Only 11 species out of 21 bear fruit, as the rest do not have time to form full-fledged seeds due to late flowering. Vegetation ends in late September-early October with the onset of the first frost. The onset of all phases, except for the duration of vegetation, is reliably influenced by species features. Phenodates of breaking of buds, growth beginning and end of shoot growth, the end of vegetation and the duration of vegetation is subject to fluctuations depending on the climatic conditions of a particular year.
Keywords: Clematis L., species, seasonal rhythm of development, phenodate
Application of artificial neural networks for identifying poorly formalized patterns in experimental become widespread nowadays. In the present work, we elaborated a prototype of a model for detection of water contamination with heavy metals. The model is a fully connected neural network (multilayer perceptron) designed by using the Python programming language and the TensorFlow software (Keras). It is intended for application in environmental monitoring of natural water bodies using chlorophyll fluorescence measurements which are considered as highly informative approach for probing photosynthetic activity in vivo and in situ. Fluorescence rise induced by application of a strong light pulse to the dark-adapted plant or algae (the OJIP transient) reflects a stepwise transition of the photosynthetic electron transport chain from the oxidized to the fully reduced state. To provide a quantitative analysis of the OJIP transient, a JIP test was introduced whose parameters describe energy fluxes through the photosynthetic electron transport chain. Our model uses OJIP transients and/or JIP-test parameters, measured in phytoplankton communities, as input data. As a result, it determines the probability of water pollution by heavy metals. In order to test the model, phytoplankton samples were taken from 9 water bodies of Pskov region and then treated with chromium and cadmium under laboratory conditions. For that, phytoplankton samples were exposed to cadmium and chromium salts (CdSO4 and K2Cr2O7) at two concentrations (20 and 50 μM) for three days, and OJIP curves were recorded and JIP-test parameters calculated at different stages of the experiment. In total, 419 curves were collected, and a whole dataset was analyzed. Results showed that accuracy of detecting the toxic effects of Cd2+ (after 2 or more hours of incubation) and Cr2O72– (after 9 or more hours of incubation) by the model achieved 90%. The highly accurate determination of the toxicity of heavy metals indicates a promising prospect for the application of machine learning technology in environmental monitoring.
Article published in number 2 for 2021 DOI: 10.25750/1995-4301-2021-2-134-141
Views: 45
Improvement of fluorometric method for polycyclic aromatic hydrocarbons determination in environmental objects
O.A. Plotnikova
Section: Methodology and research methods. Models and forecasts
The selection of suitable pre-sample preparation and analysis methods for effective monitoring of polycyclic aromatic hydrocarbons (PAHs) is essential. An important task is to control the PAHs content in various media. These substances, even at low concentrations, can pose a threat to public health, can have carcinogenic, mutagenic and teratogenic effects on living organisms. A distinctive feature of PAHs molecules is the ability to absorb energy and luminescence under the influence of UV radiation. In this regard, spectroscopic methods are promising for the PAHs determination.
The paper presents the characteristic absorption and fluorescence spectra of PAHs, and indicates their excitation and fluorescence wavelengths. An experimental study of the metrological characteristics of various spectroscopic methods has been carried out. The PAHs representative pyrene was determined by spectrophotometry and fluorimetry in solutions, as well as solid-phase fluorimetry with preliminary micellar and sorption concentration on a solid matrix. The use of aqueous micellar solutions of the nonionic surfactant Triton X–100 made it possible to enhance the analytical signals for the pyrene determination. The data obtained confirm the fact that the fluorescence method is characterized by a higher sensitivity than the method based on the registration of these substances’ absorption. The linear range of concentrations determined by the spectrophotometric method is more significant in comparison with the fluorometric methods and amounts to 14–10000 ng / ml. At the same time, the combination of preliminary micellar and sorption concentration with solid-phase fluorescence on a modified by surfactants viscose matrix made it possible to reduce the detection limit of pyrene to 0.8 ng / ml, which is especially important for the development of methods for controlling ecotoxicants contained in the environment in "trace" concentrations.