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Analysis of the cytogenetic status of children and adolescents in the living in areas with different environmental conditions

S.V. Ermolaeva, Е.О. Ivanov

Section: Social ecology

Cytogenetic status can be a marker of ecological trouble in a certain area.Cytogenetic status is determined by the frequency of micronuclei in buccal epithelial cells. The largest amount of pollutants enters the atmospheric air of Ulyanovsk and the Sengileevsky district. In the drinking water of the Sengileevsky and Cherdaklinsky districts, iron appeared to be up to 10 times higher than the MAC, manganese – up to 2.5 times. The content Pb, Zn, Ni in the soil were up to 12 times higher than the MPC. This study aims to evaluate the frequency of micronuclei in the cells of the buccal epithelium of children and adolescents living in an industrialized zone, compared with that inside children living in areas without significant anthropogenic impacts. The preparations were made according to standard methods. 1000 cells per individual were analyzed. As a control measure we used indicators of the frequency of micronuclei in the cells of children and adolescents from the Veshkaimsky and Sursky districts, in which no excess of the MPC of toxic substances was detected. The analysis was carried out in 418 people aged 9–15 years, permanently residing in the study areas. The frequency of cells with micronuclei in schoolchildren of the Zasviyazhsky district of the city of Ulyanovsk was 1.46±0.87‰ is 7 times higher than the control, in the second group (Cherdaklinsky district) 1.22±0.95‰ is 6 times higher than the control, in the 3rd sample (Sengileevsky district) 1.19±0.44‰, 5 times more than control. The index of cytogenetic disorders in group 1 – 4.18 (high risk), in group 2 – 3.42 (moderate risk), in group 3 – 4.00 (high risk). The genotoxic effect of pollutants in the environment of Ulyanovsk (Zasviyazhsky district) and Sengileevsky district was established.

Keywords: ecological safety, cytogenetic status, buccal epithelium, accumulation of cytogenetic disorders, risk groups

Article published in number 2 for 2022
DOI: 10.25750/1995-4301-2022-2-234-240

Contamination of soil cover with compounds of metals, arsenic and antimony in the area of impact of fuel and energy complex

D.G. Sycheva, N.E. Kosheleva, I.V. Timofeev

Section: Monitoring of natural and anthropogenically disturbed areas

The relevance of the study is caused by a significant amount of toxic emissions from thermal power plants (TPPs) operating on brown coal, which can precipitate from the atmosphere and accumulate in urban soils. To assess the impact of TPP emissions on soil pollution in the city of Gusinoozyorsk (Republic of Buryatia), samples were taken from the upper (0–10 cm) horizons on a regular grid. With the help of the ICP-MS and ICP-AES methods the content of Zn, As, Cd, Bi, Pb, Cr, Co, Ni, Cu, Sb, Mo, V, W, Sr, Ag were analyzed in 79 soil samples, as well as in brown coal and ash of Gusinoozyorsk TPP, whose emissions are the main source of pollution. Brown coals contain concentrations of Mo, W, Sr, Zn, V 1.5–2.9 times higher than the clarkes for coals of the world. Ash is enriched with Mo, Sr, V, Cu, Co compared to world clarkes. The ability of soils to adsorb heavy metals, arsenic and antimony is determined by their physicochemical properties: a slightly alkaline reaction, a medium loamy texture and an average content of organic matter (2.7%). The soils of the industrial operating and one-storey residential land use subzones are the most contaminated. In the first zone Cu, Ag, As, Sr, Co, V, Ni, Sb accumulate which are associated with emissions from the Gusinoozyorsk TPP and the influence of coal dust. In the second zone Sb, Cu, Sr, Zn, Ag, Cd, Pb accumulate which enter the soils with fly ash from the TPP, household waste and car emissions. Heavy metals and metalloids in the soil cover have formed several local anthropogenic anomalies of high and moderate contrast, which occupy 7% of the city’s area.

Keywords: heavy metals, metalloids, urban soils, environmental geochemistry, thermal power plant, brown coal, fly ash

Article published in number 2 for 2022
DOI: 10.25750/1995-4301-2022-2-048-055

Lithophage Alces alces L. in the protected territories of Yakutia

V.V. Stepanova, A.V. Argunov, D.I. Tirskiy, R.A. Kirillin, I.M. Okhlopkov

Section: Population ecology

Registration of moose lithophagy by means of photo traps was carried out on six salt lick in the protected territories of Southern and Central Yakutia. The duration of moose lithophagy is up to 132 minutes, with an average of 9-12 minutes. Females salted longer than males. In Southern Yakutia, half of the visits to lick occur at the darkest time of the day from 24 am to 4 am (50.5%), and in Central Yakutia from 20 am to 24 pm (42.5%). The lowest attendance of lick was observed in both cases during daylight hours from 8 am to 20 pm (5.6% and 8.6%). In both parts of the range, females are more likely than males to visit lick at dawn from 4 am to 8 am. The highest activity of salting in both areas is observed in June: in Southern Yakutia – 5.7 moose per day, in Central Yakutia – 2.1 moose per day. This circumstance is explained by the period of calving in females and the development of horns in males. For the entire observation period, the average number of visits per day by animals was: in Southern Yakutia – 3.7 (in the period from May to July), in Central Yakutia – 1.0 (in the period from June to August). In southern Yakutia, there is a sharp increase in male visits to lick in the month of June, which falls sharply in July. In Central Yakutia, this is not observed. The sex ratio of individuals who visited salt lick was: in Central Yakutia, males – 60.9%, females – 39.1%; in Southern Yakutia, males – 45.7%, females – 54.3%. The percentage of calves was: in Southern Yakutia – 10.2%, in Central Yakutia – 30.3%. In Southern Yakutia, females with moose calves accounted for 3.4% of all visits, and in Central Yakutia – 11.2%.

Keywords: moose, salt lick attendance, duration of salt licking, lithophage time distribution

Article published in number 2 for 2022
DOI: 10.25750/1995-4301-2022-2-138-144

Dynamics of vegetation of the phytomeliorated deflation zone on the territory of the Republic of Kalmykia

L.P. Rybashlykova

Section: Remediation and rehabilitation

The state-of-the-art of the vegetation cover and plant communities composition of the restored phytomeliorated deflation zone has been studied. Forest reclamation arrangement of the “Green zone – Komsomol’skij village” deflation zone was carried out in 1998 in a destructive-accumulative zone by continuous planting of Calligonum aphyllum without preliminary soil preparation. Tree-planting machine MLB-1 was used for these purposes. Forb-cereal and white wormwood-cereal communities with a projective cover of 65% is currently dominated in the vegetation cover of the lower grassy tier (age is 20–23 years). The species composition has stabilized to 10–15 years. About 30 species have been included in the species composition. The formation and stabilization of phytocenosis occurs under the protection of Calligonum aphyllum planting for 18–20-years period of succession. Conditions for the introduction of species, the growth of which required a certain level of soil fertility, as well as more comfortable microclimate than on degraded soils and open sands, have been created in the same period. There are changes in the ecobiomorphic composition of phytocenosis, a decrease in the share of annual species, an increase in the number of forbs with an increasing in the duration of successions. It was found that progressive successions of the site vegetation cover inhibit by overgrazing and pyrogenic factor. Under the influence of overgrazing in the species composition in a competitive environment, species are replaced by other species in approximately equal relation. There is a decrease in the feed value of phytocenoses with the development of monocultures (low-value plant species) The obtained data on the flora, abundance, and structure of vegetation cover demonstrate the current state in the "exhausted" centers of deflation in the pastures of the Republic of Kalmykia.

Keywords: arid zone, land degradation, phytomelioration, succession, plant community

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Article published in number 2 for 2022
DOI: 10.25750/1995-4301-2022-2-152-158

The chemical composition of atmospheric precipitation, throughfall and surface waters in the middle taiga deciduous stands of post-cutting origin

Т.А. Pristova

Section: Chemistry of natural environments and objects

The chemical composition of atmospheric precipitation (rainfall), throughfall and surface (stream) waters are studied. The studies were conducted in the Knyazhpogost district of the Komi Republic (62̊18’ N 50̊55’ E). Studies were conducted on the terrain in which from 1970 to the 1990. spruce forests were cut down and now represented by deciduous and deciduous-coniferous forest of different ages, which are formed after natural reforestation. Water samples were taken monthly at constant points for 2 years: in a stream, atmospheric precipitation (rainfall), throughfall the crowns of birch, aspen, spruce and between crowns in 10-15 replicates. The chemical composition of the waters is transformed in the series: atmospheric precipitation (rainfall) – throughfall – stream. It is established that when throughfall the crowns of trees, the content of most components of the chemical composition of atmospheric precipitation increases. The chemical composition of the throughfall is affected by the age and tree composition of the stand. The studied natural waters are slightly acidic, low salinity, hydrocarbonate class and differ in hydrochemical type. A series of decreasing ion concentrations in atmospheric precipitation: HCO3->SO42->K+>Ca2+>Cl-, throughfall of birch-spruce young forest: HCO3->K+>Ca2+>SO42->Cl-, throughfall aspen-birch stand: HCO3->K+>Ca2+>PO42->Cl-, in the stream waters: HCO3->Ca2+>Mg2+>K+>Cl-. As the mineralization increases, the studied waters are arranged in a row: atmospheric precipitation (rainfall) < throughfall of birch-spruce young forest < throughfall of aspen-birch stand

Keywords: middle taiga zone, deciduous forests, chemical composition of precipitation and water stream

Article published in number 2 for 2022
DOI: 10.25750/1995-4301-2022-2-063-069

Specifics of biogeocenoses restoration during overgrowth of coal-mine dumps of the Gorlovsky basin

A.N. Bespalov, N.A. Sokolova, D.A. Sokolov

Section: Remediation and rehabilitation

Due to the increasing volume of anthracite production in the Novosibirsk region, the area of disturbed territories is also increasing. In this regard, the problem of restoring ecosystems on dumps is acute. The vast majority of researches on the restoration of man-made ecosystems is aimed at studying any one of their components. The purpose of this work was a comprehensive assessment of the specifics of recovery of waste dumps of the Gorlovsky anthracite deposit (Novosibirsk region). It included a study of the development of soil and vegetation cover, as well as an assessment of the biodiversity of soil invertebrates (on example of ground beetles). It was revealed that three types of soil are formed in the composition of the soil cover of the studied objects – initial, organo-accumulative and turf embryozems. The soil-ecological condition of sample areas was evaluated quantitatively in bonus points, taking into account the topography of the dumps (leveled and hilly areas), the density of the top layer of soil (0-10 cm), and the stage of evolution of young soils. It is shown that the succession of vegetation cover proceeds in the direction from pioneer groups to closed phytocenoses simultaneously with the development of young soils. The population of ground beetles was studied. 43 species belonging to 17 genera were found. The interaction between ecosystem components is studied. Using statistical methods, it is shown that along with the restoration of plant communities and the formation of soil cover on the studied dumps, both the number of species of ground beetles and the indices of diversity increase. Species are replaced by specialized ones, and the number of dominants increases. The species diversity of ground beetles increases syngenetically to the improvement of the soil-ecological condition.

Keywords: coal-mine dumps, embryozems, soil-ecologic condition, mesofauna, Carabidae

Article published in number 2 for 2022
DOI: 10.25750/1995-4301-2022-2-159-164

Problems of legal regulation of commercial use and conservation of fishery resources

A.M. Tortsev

Section: Social ecology

Fish, aquatic animals and plants are an important part of ecosystems. They are involved as a resource in economic activity by humans. The fishery resources are an important source of income and welfare of coastal communities and a driver of the socio-economic development of the regions. Balanced long-term management and conservation of fisheries resources for future generations is the basis for regulating commercial activities. Fishery resources are an element of direct and indirect economic activity of entrepreneurs who carry out fishing and use the shores of water areas. The study of the legal regulation of the use of fishery resources, identifying problems and suggesting ways to solve them is the aim of the research. The methods of content analysis, systematization and structuring of information were used in the research. Analysis of legal norms and scientific publications, interviews with economic entities were carried out. The study of the regulation of economic activity revealed a multiplicity of problems associated with the interaction of various branches of law and departmental documents in the field of fishery resources. We have prepared proposals for the development of regulatory standards, including: the creation of intersectional expert groups with the involvement of specialists in various fields and stakeholders, verification of the developed regulatory standards and improvement of contract law in the use of fishery resources. Thus, harmonization of economic regulation will remove administrative barriers to economic growth and preserve natural resources at the same time.

Keywords: fishery resources, commercial activity, legal regulation

Article published in number 2 for 2022
DOI: 10.25750/1995-4301-2022-2-216-221

Formation of a green production management model

A.A. Malyshev, N.N. Solodkov, N.A. Korobkova

Section: Ecologization of industry

The article deals with the problem of managing green production at various levels: federal, regional and enterprise level. The approaches of foreign and Russian authors to the disclosure of the essence of the concepts of “ecologized production”, as well as “green production”, various methods of studying this problem are analyzed. A management scheme for green production is presented, as well as the results of a study of the management structure at various levels. The structure of green production management carried out by federal and regional executive authorities in accordance with the Law “On Environmental Protection” was analyzed, which made it possible to systematize the tasks and functions of green production management bodies at the federal, regional and local levels. The main functions and principles of managing the system of green production are considered. An analysis of emissions of pollutants into the atmosphere in dynamics by years is given, investments in fixed assets aimed at protecting the environment and rational use of natural resources are analyzed. A correlation was found between all levels of management in terms of the dynamics of pollutant emissions and investments in fixed assets. A study has been conducted, which indicates that, firstly, the insufficiently efficient use of funds allocated for environmental protection; secondly, about the imperfection of the mechanisms for managing green production at its various levels. A model for managing green production at three levels (federal, regional and enterprise level) is proposed, including functions, principles, methods and tools. The model of green production is based on the following functions: stimulation associated with the creation of motivation for enterprises to conduct environmental activities; redistribution of environmental activities, products and pollution between manufacturing enterprises; regulation of environmental activities, products and pollution of enterprises and organizations; a supervisory function related to environmental activity, product and pollution control activities; accumulating function, which is to create the necessary financial and environmental reserve. This model can be used by the environmental services of various enterprises, the Ministry of Natural Resources and Ecology when developing a development strategy, the Department of Economics, Innovation and Scientific and Technical Policy to develop innovative concepts of environmental p

Keywords: green production, greening production, management in the field of environmental management, management of environmentally friendly production, management system

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Article published in number 2 for 2022
DOI: 10.25750/1995-4301-2022-2-093-100

Comparative evaluation of nutrient media for the cultivation of micromycetes of the genus Trichoderma

P.A. Starikov, L.I. Domracheva, S.G. Skugoreva

Section: Methodology and research methods. Models and forecasts

Fungi of the genus Trichoderma are one of the most used and promising objects of biotechnology. Therefore, among the tasks in the practical application of this micromycete in the future is the isolation of new active strains from the environment and the expansion of the range of culture media used for trichoderma. In this work, we studied the dynamics of vegetative growth of fungi of the genus Trichoderma from various ecological niches. We used isolates from the surface of decaying wood, fruiting bodies of polypores, as well as a strain from urban soil, which was cultivated for 10 years on a medium with sevilen polymer. Cultivation was carried out at 23°С on 5 agar nutrient media, namely, on bean agar, potato and potato-sucrose medium (20 g of sucrose per liter), on the classical Czapek medium, and also on decoction of Fomitopsis pinicola (200 g of fruiting bodies per 1 l of broth). The maximum rates of growth and formation of conidia were observed during surface cultivation of these micromycetes on bean agar and potato-sucrose medium. On the same media of Trichoderma sp., sevilen destructor showed a significant lag in the rate of linear growth (7.55–8.28 mm/day) in comparison with the other studied strains (19.72–24.32 mm/day) which may be explained by profound metabolic changes during long-term cultivation on media where the only source of carbon is a synthetic polymer. The least suitable substrate for cultivation of all studied isolates of Trichoderma spp. turned out to be an agar decoction of polypore, the growth rate on which was 3.55–12.06 mm/day. In all variants of the cultivation of fungi of the genus Trichoderma, the linear growth rate was constant throughout the entire process of cultivation.

Keywords: Trichoderma, micromycetes, cultivation, culture media, growth rate, sporulation

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Article published in number 1 for 2022
DOI: 10.25750/1995-4301-2022-1-044-049

Comparative evaluation of the efficiency of solid and liquid dispersants in simulation of oil and oil product spills

S.G. Litvinets, E.A. Martinson, S.M. Kuznetsov, E.O. Zadorina, O.A. Novikova, V.G. Komosko, A.V. Nikolaeva, M A. Troshin, M.T. Gaysin

Section: Ecologization of industry

The effectiveness of the «Dimex» solid dispersant developed in the interests of PJSC «Transneft» for cleaning the water surface from films of oil and oil products through dispersion, including in winter (ice) conditions, has been studied. Liquid dispersant «Finasol OSR 52», manufactured by Total Fluides, France, approved for use in Russia, was used as a reference drug. The possibility of using the modified Warren Spring Laboratory test as a method for evaluating the effectiveness of dispersion for solid dispersants is shown. Under conditions of a wide range of changing parameters, including: salinity of model seawater, water temperature, presence of sludge and broken ice, dispersant: oil ratio, type of oil product, the high efficiency of the solid dispersant «Dimex» was shown, which was for medium sulphurous oil with a density of 870.0 kg/m3 – from 35.69% to 60.67%, for light, sweet oil with a density of 844.0 kg/m3 – from 39.21% to 58.70%, for diesel fuel with a density of 830.8 kg/m3 – from 50.73% to 72.21%, for kerosene with a density of 785.5 kg/m3 – from 52.34% to 60.45%, for gasoline with a density of 739.2 kg/m3 – from 40.41 % to 49.82%. The obtained values are comparable with the values for the «Finasol OSR52» liquid dispersant registered in the territory of the Russian Federation, which makes it possible to recommend the «Dimex» solid dispersant for use in the elimination of emergency oil and oil products spills in offshore areas in the North.

Keywords: solid dispersant, liquid dispersant, dispersant activity, oil and oil products, gasoline, kerosene, diesel fuel, dispersant activity assessment method, WSL-test

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Article published in number 1 for 2022
DOI: 10.25750/1995-4301-2022-1-115-123

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