Population and biological preconditions for the cattle retroviruses` expansion
D. Abdessemed, E. S. Krasnikova, V. A. Agoltsov, A. V. Krasnikov
Section: Social ecology
This research was aimed at studying of population and biological aspects of cattle retroviruses’ expansion, such as
breed and age of animals, form of head ownership, retroviruses biology.
Bovine leukemia virus
(
BLV
) and
bovine immuno
-
deficiency virus
(
BIV
) are retroviruses which cause chronic incurable diseases of cattle. These agents have a phylogenetic
relationship with similar pathogens in humans. There is a possible danger of viral entry to humans through the consump
-
tion of infected foodstuffs. 773 blood samples from Black-and-White, Holstein, Simmental, Kazakh White-headed and
crossbred cattle of different districts of the Saratov region were analyzed by polymerase chain reaction (PCR). Studies
reveal that
bovine immunodeficiency
and
bovine leukemia viruses
are spread widely in cattle of the Saratov region: 30.5%
and 39.8% on average, respectively. The infection rate varies considerably depending on age-sex group affiliation and
cattle ownership.
BIV
and
BLV
infection rates increase with the animals age, especially among farm herd. Significant
epizootic feature of retroviral infections in cattle in the Saratov region is a high level of retroviral coinfection – 25.2%
on average. The clinical complications of
BIV
infection, confirmed by laboratory studies, were most frequently recorded
in cattle aged 5–10 years. For analysis of diagnostic accuracy of serological and molecular genetic methods for enzootic
bovine leucosis diagnosis, 271 cattle blood samples were studied by PCR and AGIDT (agar gel immunodiffusion test) in
parallel. The comparative analysis shows that diagnostic efficiency of AGIDT in comparison to PCR is 30.8%. Taking into
account the retroviruses biological features, the PCR assay can be recommend as a screening method for
BLV
-infection
revealing, especially when the imported cattle are quarantined at the place of keeping. In herds with high
BLV
-infection
level, cattle should be tested for
BIV
presence to stop the cattle retroviruses’ expansion.
Keywords: polymerase chain reaction, agar gel immunodiffusion test, retroviruses, enzootic bovine leucosis, bovine immunodeficiency, expansion, diagnostics
Article published in number 3 for 2018 DOI: 10.25750/1995-4301-2018-3-116-124
Views: 13
Formation and assessment of the phytoregulatory potential of multispecies biofilms based on Fischerella muscicola
L. I. Domracheva, I. G. Shirokikh, E. V. Tovstik, S. G. Skugoreva, E. N. Reznik
Section: Chemistry of natural environments and objects
The results of experiments on the study of the features of the formation of biofilms on the basis of two- and threecomponent associations of microorganisms with the participation of Fischerella muscicola 300 cyanobacteria, Streptomyces wedmorensis streptomycete 38.11 and Fusarium avenaceum 7/2 micromycete are presented in the article. When microorganisms are introduced into sterile soil, over the three months of exposure to light, in variants with a monoculture of cyanobacteria and with a three-component association, growths (biofilms) with a population density of a phototrophic component exceeding 50 million cells/cm2 are formed. In biofilms formed by two-component associations, cyanobacteria experienced inhibition by partners, reducing the number of cells 2.4–7.0 times, and the length of filaments 3.6–7.0 times, depending on the nature of the associate. The growth of the fischerella was limited by the streptomycete culture to a greater degree than by the fungus. In ground biofilms formed on the basis of cyanobacteria Fis. muscicola, the phytopathogenic fungus F. avenaceum, in the saprotrophic phase of development, is able to unite into single structured complexes with other participants in the multi-species consortium of microorganisms, including their antagonists. Aggregation of cells in biofilms is associated not only with the isolation of polysaccharide mucus cyanobacteria, but also with the creation of a net-thread structure of growth due to threads of cyanobacteria and mycelium of heterotrophic partners, the total length of
which can reach values exceeding 400 m/cm2. The development of such biofilms on the soil surface significantly increases its ability to retain moisture and prevent erosion. The two-component association of cyanobacteria and streptomycete
can be considered as promising for biocontrol of fusarium infection. At the same time, under specific conditions of the rhizosphere, the nature of the interaction of the cultures of microorganisms under investigation may differ from that
described for sterile soil lacking root exudates. Nevertheless, in experiments with the inoculation of Triticum aestivum L. seeds, it has been established that the two-component association of Fischerella muscicola + Streptomyces wedmorensis in
perspective can be used as a biological agent to limit the development of fusarium infection in the higher plant rhizosphere.
Keywords: cyanobacteria, streptomycetes, micromycetes, biofilm, density of populations, rhizosphere, microbial inoculation
Article published in number 2 for 2018 DOI: 10.25750/1995-4301-2018-2-117-124
Views: 72
Degradation of Acid Orange 7 in a persulphate-bisulfite system under visible light
Heng Zhong, Jia Fang, Lei Sun, Hui Zhao, Aihua Xu, Dongsheng Xia, A. V. Nevsky
Section: Nature management and natural resources consumption
Textile industry is one of the most significant manufacturing sectors that produce large volumes of highly polluted and toxic wastewater. Dyes represent an important part of waste effluents. In this study, the degradation of Acid Orange 7 dye (AO7) in a persulfate-bisulfite (PS-BS) system under visible (Vis) light (wavelength more than 420 nm) was performed. All batch experiments were conducted in a 100 mL undivided glass beaker containing 50 mL solution at about 25 оC without irradiation or under irradiation by a direct exposure visible light power supply (CHF-XM-500W) from
Beijing Trusttech Co. (China). After the desired amounts of AO7 and PS in 50 mL of the aqueous solution were added into the reactor, the reaction was initialized by adding BS. To monitor the degradation process of AO7, solution samples were taken out at given reaction time intervals and measured immediately on a METASH UV-5500PC spectrophotometer under the maximum absorption wavelength (484 nm) to record the temporal UV-vis spectral variations of the dye. The related reaction progress was monitored using the UV-vis absorption spectra of AO7 in terms of the percentage of
degraded azo-dye. The activities of different systems including BS/Vis, PS/BS, PS/Vis and BS/PS/Vis were evaluated. It was found that the efficiency of PS/Vis system was negligible, and almost 29 and 40% of AO7 was oxidized by BS/Vis and BS/PS within 30 min, and it was further improved to almost 94% within 30 min by BS/PS/Vis system at room temperature. Various operational parameters, such as PS concentration, BS concentration and initial pH were investigated to optimize the process.
Article published in number 2 for 2018 DOI: 10.25750/1995-4301-2018-2-113-116
Views: 23
Some aspects of aluminum detoxifying in plants: phytotoxic and genotoxic effects
B. I. Synzynys, L. N. Ulyanenko
Section: Ecotoxicology
The separate and combined impacts of aluminum, manganese and silver ions on onion (Allium cepa L.) have been studied. The experiments have been performed in several series with different solutions of metal salts: AlCl3 • 6H2O,KMnO4, MnCl2 • 4H2O for the first and second series and Al(NO3)3 and AgNO3 – in third series. The ion concentrations of all studied metals amounted to 1 MPC according to the Russian health legislation and were 0.5 mg/L for Al,0.1 mg/L for Mn and 50 mg/L for Ag. The phytotoxic effect and genotoxic effect (such as mitotic index and the frequency
of chromosome aberrations) were estimated. It was showed that aluminum ions promote negative processes in all our experiments. We found that manganese in combined presence in solution with aluminum reduced the phytotoxic and genotoxic effects aluminum on Allium cepa: the coefficients of antagonism calculated for frequency of aberrant cells and mitotic index are equal to 0.2. We have the same tendency for the silver ions. Taking into account our previously obtained data (the reducing of genotoxic effect of aluminum ions by iron ions) it is possible to conclude about similar mechanism for manganese (regardless of valency) and silver. Thus our results demonstrated this unique and at the same time universal mechanism of interaction between two metals (aluminum and other) and their detoxification effect in plant. We anticipate our assay to be a new starting point in investigation of detoxification mechanisms for heavy metals in plants: it is found that a combination of several metals reduces the negative action of each of them.
Keywords: aluminum, manganese ions, silver ions, barley, separate and combined action, phytotoxicity and cytogenotoxicity of aluminum, antagonism indicies
Article published in number 2 for 2018 DOI: 10.25750/1995-4301-2018-2-107/2-112
Views: 27
Comprehensive chemical-toxicological research of copper(II) sulfate solutions containing reduced glutathione
E. I. Lyalina, A. I. Fokina, T. Ya. Ashikhmina, A. S. Olkova, E. V. Beresneva, L. V. Darovskikh, A. S. Yarmolenko
Section: Ecotoxicology
The composition and toxicological properties of copper(II) sulfate solutions containing reduced glutathione (GSH)have been studied. It was found that complex compounds Cu2+ with GSH are formed in the solution in the Cu(II):GSH molar ratios 1:1, 1:2, 1:3, 1:4. Simultaneously with the formation of complexes in solutions, oxidation-reduction reactions occur, accompanied by the formation of active forms of oxygen and oxidized glutathione (GSSG). All the described processes lead to a decrease in the active concentration of Cu2+. The toxicological properties of solutions with a molar ratio of Cu(II):GSH 1:1, 1:2, 1:4 were determined. For this purpose, test objects of different systematic accessory were used: cyanobacterium Nostoc linckia 271, crustaceans Daphnia magna Straus, infusoria Raramecium caudatum Ehrenberg and bioassay “Ecolum” . The chemical composition of solutions was correlated with toxicity. Reduced glutathione has a protective effect on daphnia, which is most pronounced in the variant 1Cu(II):4GSH. However, the level of viability of cyanobacteria, in contrast to hydrobionts, decreases in the 1:1 → 1:2 → 1:4 range. The decrease is due to the bioaccumulation of copper, which increases with the increase in the fraction of reduced glutathione. Such an effect may be formed
by complex compounds, which is consistent with the literature data. For D. magna, such a relationship is not observed, the resulting compounds and reduced glutathione do not affect the accumulation of metal.
Keywords: reduced glutathione, copper(II) ions, bioassay, active forms of oxygen
Article published in number 2 for 2018 DOI: 10.25750/1995-4301-2018-2-101/2-107/1
Views: 21
Assessment of soil contamination in the recreational areas of the city by the industrial compounds of heavy metals and arsenic
T. A. Trifonova, A. A. Podolets, O. G. Selivanov, A. A. Martsev, A. A. Podolets
Section: Ecotoxicology
The article presents the results of determination of total content of heavy metals (HM) and arsenic in urban soils of recreational areas in the city of Kolchugino of Vladimir Region. The most polluted recreational areas of the city that are
close to the industrial area and in the Central area. According to a spatial correlation analysis, the elements Pb, As, Zn,Cu, Ni, Co and Cr contained in the soils of the city are in approximately equal ratio in all reference areas that may indicate the joint intake of these pollutants. With the aim of obtaining justified results of anthropogenic accumulation of HM and arsenic in the soil, an analysis of ratio of the content of pollutants in the upper layer to its content in the bottom was conducted using the radial differentiation coefficient (R). It is established that the pollutants are concentrated mainly in the upper layers of the soil, and their accumulation has an anthropogenic character. To identify priority of pollutants, the index of accumulation was used, which decreases in the series: Cu > Zn > Pb > Ni > As > Co = Cr > V > Sr. On the calculated level of soil pollution by separate pollutants, the the excess over maximum permissible concentration was
revealed in the following series: Zn > Cu > As > Pb > Ni. The highest values of the hazard coefficient were noted in the area of the plant “Kolchugtsvetmet”. The correlation analysis between the pollutant concentrations revealed statistically
significant positive dependences (p < 0.05) between Pb, As, Zn, Cu, Ni, Co and Cr. Based on this, it can be assumed that these elements enter the soil of the city from one source.
Keywords: heavy metals, gray forest soil, recreational areas, the coefficient of radial differentiation, the rate of accumulation, the hazard ratio
Article published in number 2 for 2018 DOI: 10.25750/1995-4301-2018-2-094-101/1
Views: 53
Technology for conversion of whey into organic-mineral fertilizers with amino acids
Yu. N. Terentyev, N. V. Syrchina, T. Ya. Ashikhmina, А. V. Sazanov, M. L. Sazanova, V. A. Kozvonin, D.V. Petukhov
Section: Ecologization of industry
Rational processing of whey is one of the most important problems of the dairy industry. Less than 50% of this waste is processed in Russia. Unprocessed whey is discharged into the sewage systems, which significantly increases the degree of contamination of the process waste water. Basically, processing of whey in commodity products is limited by economic factors. At the enterprises of small and average capacity high costs of introducing processing technologies do not pay off proceeds from realization of the received products. The authors of the research have used the method of cryogenic concentration (freezing) which provided the complete conservation of bioactive substances (proteins, vitamins,enzymes) in whey. Proteins of the received concentrate were subjected to enzymatic hydrolysis to free amino acids. The
mineral salts complex (which contains plant-available forms of nitrogen, phosphorus, potassium, magnesium and trace elements) was dissolved in the hydrolysate. As a result, the researchers have got a marketable product – liquid organicmineral
fertilizer with natural amino acids. Whey-fraction that remained after cryogenic concentration contained no more than 1.2% of dry substances. For its removal there was used glauconite-containing efel as sorbent (the waste of phosphorite ore enrichment, it is a quartz-glauconite sand containing up to 45% glauconite). The waste sorbent was dried up and used as the second commercial product – solid organic-mineral fertilizer (S-OMF). The S-OMF included only natural ingredients, which made this product suitable for use as a fertilizer in natural farming systems.
The simplicity and high market value of the received marketable products make it economically viable to introduce the proposed technology into practice. The calculated payback period of the offered technology at the enterprises of a
mean power is less than two years.
Article published in number 2 for 2018 DOI: 10.25750/1995-4301-2018-2-087-093
Views: 14
Modification of the sludge from soda production for producing oil sorbents
E. V. Kalinina, I. S. Glushankova, L. V. Rudakova
Section: Ecologization of industry
At present, the volume of accumulated sludge from soda production is more than 50 million tons and the issue of their utilization is topical. The paper presents the results of studies on the production of oil sorbents based on soda production
sludge, the main component of which is calcium carbonate. Reagent modification of sludge from soda production allows obtaining materials that have sorption activity to oil and petroleum products. It is established that the oil capacity of
samples of initial sludge of soda production is 0.95 for gasoline; on engine oil – 0,9; for oil – 0.75 g/g. Reagent treatment of soda production sludge by sodium methylsiliconate or potassium methylsiliconate increases the petroleum capacity
on gasoline by 30%; on engine oil – by 80%; for oil – by 73%. Obtained sorbents can be used for liquidation of oil and oil product spills from solid surfaces. For the liquidation of oil and oil product spills from the surface of water bodies, soda
production sludge of less than 0.1 mm fraction obtained by modification with sodium methyl siliconate or potassium methylsiliconate with a working dose of 1.5–1.8% by weight of active substance and heating to a temperature of 90 °C may be recommended. Based on the results of the research, two environmental objectives can be achieved: new oil sorbents are obtained and a method for the utilization of sludge from soda production is proposed. The cost of oil sorbents based on waste will be low, due to the zero cost of the raw materials – waste products of soda production.
Article published in number 2 for 2018 DOI: 10.25750/1995-4301-2018-2-079-086
Views: 11
Thermotechnical properties of municipal solid waste depending upon component and fractional composition
S. V. Polygalov, G. V. Ilinykh, V. N. Korotaev
Section: Ecologization of industry
Thermotechnical properties of municipal solid waste (MSW), in particular their moisture, ash content and heating value, determine the effectiveness of methods of thermal utilization of waste, interest in which is currently growing in Russia. The MSW heterogeneity leads to difficulties in determining their thermotechnical properties and causes the urgency of carrying out complex researches of MSW fractional and component composition with the isolation of sufficiently homogeneous components, the selection and laboratory researches of samples of such components. The article presents an approved methodological approach to MSW analysis, which includes full-scale field investigation of MSW component and fractional composition and analysis of their moisture and ash content in a laboratory with the subsequent calculation of the thermotechnical properties of the MSW flow as a whole. A distinctive feature of this approach is the provision of representative samples of MSW and individual components, consideration of seasonal and weekly fluctuations in waste composition, as well as the allocation of a large number of MSW components and separate investigation of their moisture and ash content in fractions of different sizes. Moisture and ash content of individual components within the same category differ significantly from each other and depend on the component’s material, properties and size. Heating value an individual component category depends on the component composition within the category and vary with the same water and ash content of the individual components. The obtained results allow modeling of MSW composition, heating value and other properties after different treatment technologies, including mechanical, manual and optical sorting, and
select technologies and equipment for achieving specified waste quality parameters.
Keywords: component composition of waste, fractional composition of waste, moisture, ash content, heating value
Article published in number 2 for 2018 DOI: 10.25750/1995-4301-2018-2-070-078
Views: 11
Assessment of the composition and the microstructure of aluminiumcontaining waste for the substantiation of the directions of its use
A. A. Martynova, G. M. Batrakova
Section: Ecologization of industry
Complex recycling of industrial wastes involves the extraction of valuable components and the use of waste as secondary material resources. Implementation of resource-saving technologies reduces the anthropogenic load on the environment.
In the process of thermal destruction of calorific materials, waste with a high content of aluminum oxide is generated. In order to determine the directions of usage of alumina-containing waste the initial study of its composition and structure
was made. The methods of scanning electron microscopy and quantitative physicochemical analysis were used. The following samples were studied: construction samples with waste additives (15 and 30%), and a series of samples purified
with mineral acids and water. The mineral composition of the alumina waste corresponded to the requirements for raw materials for use as a reinforcing
additive in cement mixtures, heat-resistant concretes and for the manufacture of binders. Compaction of the structure by spherical particles Al2O3 affects the strength characteristics of gypsum binder samples. A comparison is made between
the physico-mechanical characteristics of samples with different waste content and control sample of gypsum. A study of the microstructure and element analysis of waste, purified by mineral acids, was made. The analysis showed an inhomogeneous composition of impurities. Treatment of waste with acids and water is effectively remove impurities, at the same time mass content of aluminum in the purified samples is increasing.
The results of evaluation of waste structure and composition can be used for explanation of materials formation with new structure on aluminum-oxygen basis and ceramic materials formation that can be manufactured using this type of waste.
ISSN 1995-4301
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