Biodegradation of polymeric composite materials by microscopic fungi
E. Kh. Sakaeva, Yu. V. Kulikova, L. V. Rudakova
Section: Ecologization of industry
The objects of research were polymer composite materials (PCM) based on various reinforced materials and binders:
fiberglass, organoplastics and carbon plastics. The program of experimental studies included three stages. At the first
stage, studies were conducted to identify groups of microorganisms involved in the biodegradation of PCM. The second
stage of the research is devoted to determining the generic and species belonging to microorganisms obtained in the first
stage. At the third stage, the efficiency of the process of biological destruction of PCM by isolated groups of microorganisms
was evaluated. Determination of groups of microorganisms involved in biodestruction was carried out by seeding
on liquid nutrient substrates. Fungi resistance was determined in accordance with GOST 9.049-91.
The main groups of microorganisms developing on the surface of composites are representatives of the genus Peni�
cillium (P. chrysogenum, P. funiculosum, P. cyclopium) and the genus Aspergillus (A. niger, A. terreus, A. oryzae). The
evaluation of the fungi resistance showed that the PCM are non-resistance, so the growth of the fungi is estimated at
4‒ to 5 points. The epoxy matrix is the least fungi resistant, whereas the phenol-formaldehyde matrix more resistant to
fungal destructors. The results of the study shows the advisability of biodegradation technology with microscopic fungi
using for the utilization of polymeric composite materials based on epoxy and phenol-formaldehyde resins reinforced
with glass, carbon or organic fibers.
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-061-068
Views: 36
Study of migration and transformation of greasing and cooling fluids in the soil of urbanized area
L. M. Shuklina, G. M. Batrakova, T. N. Nurislamova
Section: Ecologization of industry
Relevance of the work is determined by the requirements of ecological safety in the industrial and civil construction
in areas with identified technogenic soils’ pollution. The paper considers the use of chromatography-mass spectrometry
in a study of polluted soil in order to identify transformation products under natural conditions of multicomponent formulas
of lubricating fluids. The identification and search by mass spectra of the component composition of the industrial
oil transformation products was carried out, a marker compound is proposed. The possibility of structure and element
composition identification on the basis of registration of mass spectra of chemical compounds is shown. The search for
characteristic ions of organic compounds was carried out using a bank of the mass-spectral data library. The characteristic ions (basic and sustaining) describing the structure of chemical compounds and the chromatographic retention time
are determined. To assess the migration of the marker compound, a quantitative determination of the marker compound
was made by capillary gas chromatography in air and polluted soils samples. Soil samples at the bottom of the vessel,
samples of oil-retaining tissue and filtrate from the pallet were examined.
The results of the research are aimed at justifying the method for assessing the contamination of industrial sites
with lubricating fluids, taking into account the transformation of the organic pollutant and recommendations for the
recultivation of polluted technozems.
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-069-077
Views: 36
Production of environmentally friendly building materials with use of pulp and paper sludge
E. S. Shirinkina
Section: Ecologization of industry
The paper presents the research in the field of using pulp and paper sludge as a resource in the production of environmentally-
friendly building materials. During the analysis of scientific and technical information, it was found out
that pulp and paper sludge, containing cellulose fiber and secondary sludge, can be used as an additive in the production
of composite materials, insulation boards, light aggregates for concretes and combustible additives for production of
ceramic bricks. In the laboratory, the possibility of obtaining a ceramic brick with addition of pulp and paper sludge in
an amount of 4 and 8% by weight was researched. It was found that when pulp and paper sludge is introduced into the
ceramic mass, the density of the bricks samples decreases, linear air shrinkage decreases to 2.7% (for samples with pulp
and paper sludge addition in quantity of 4% mass), and 9.1% (for samples with pulp and paper sludge addition in quantity
of 8% mass) in comparison with control samples without pulp and paper sludge addition, fire shrinkage decreases from
5.1% to 3.65% and 2.06% for samples with pulp and paper sludge content 4% and 8% mass respectively. Compressive
strength of researched samples with pulp and paper sludge content significantly increases (to 90.2%) in comparison
with the control samples, which is due to the release of additional thermal energy during the combustion of the pulp and
paper sludge in the ceramic mass while the brick firing process. It has also been established that the bending strength
of researched samples is reduced in comparison with the control samples, but with an increase of firing temperature to
1070 оC, an increase in bending strength has also been achieved. A comparative analysis of the obtained data on the
strength of ceramic brick samples with the requirements of National State Standard showed that when the pulp and
paper sludge is introduced into the ceramic mass in an amount of 4–8% by weight, as a combustible additive, it is
possible to obtain solid ceramic brick suitable for building.
Keywords: pulp and paper sludge, secondary sludge, primary sludge, cellulose fiber, combustible additive
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-078-085
Views: 36
Biomonitoring capabilities of microorganisms when assessing the degree of toxicity of synthetic surfactants
L. V. Kondakova, L. I. Domracheva, V. S. Simakova
Section: Ecotoxicology
High sensitivity of the two species of cyanobacteria (Nostoc paludosum and Fischerella muscicola) as test-organisms
by means of stating their hydrogenase activity with the tetrazolium-topographical method was proved; presence of
formazan in cyanobacteria living cells served as a marker. It was stated that decrease in specious composition of algae
and cyanobacteria can serve as a bioindication sign of synthetic surface-active substances in the environment. Formazan accumulation decreased considerably under the influence of car wash of three trade-marks. When cyanobacteria cultures
are incubated in solutions of car shampoos with a recommended dose concentration, the number of viable cells drops
sharply. Bioassay with the help of bacilli was carried out by means of bacterial suspension inoculation on nutrient agar
with preliminary added sodium lauryl sulfate (SLS). The toxicity criterion consisted in decreasing number of the grown
bacilli colonies in experimental variants. It is shown that the degree of toxicity of SLS can be tested using bacteria of
the genus Bacillus, judging from decrease of the percent of soil-balls encrusting with bacteria of the genus Azotobacter.
The representatives of Xanthophyta and Eustigmatophyta were the most sensitive to soil pollution with SLS. The
number of algae species was the most in the control variant and in the variant with 0.5 recommended dose of SLS. The
results showing negative influence of the tested synthetic surface-active substances on microorganisms with different systematic
characteristics proved the fact that it is necessary to carefully use car wash and to avoid car wash getting into soil.
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-086-089
Views: 36
Applying songbird population dynamics models to conservation biology needs
A. L. Podolsky
Section: Population ecology
Proper understanding of the reproductive biology traits and population dynamics patterns of declining songbird
species is crucial for ensuring their effective protection and recovery. Metapopulation dynamics may cause the extinction
of local populations in some landscape patches regardless of the habitat quality and undertaken conservation measures.
At the same time, the source-sink type of the population dynamics could saturate lower quality habitat patches with dispersing
individuals from the population sources. Hence, poorer quality habitats presumed to yield population sinks could
eventually maintain population sources. Consequently, an effective recovery strategy for declining species should include
high quality suitable habitats along with some poorer quality patches in the regional network of protected natural areas.
I developed the mathematical model for songbird reproductive strategy based on the case study of my three-year
field research conducted on the Ovenbird (Seiurus aurocapilla L.) in the Great Smoky Mountains National Park (U.S.A.).
Breeding Bird Survey detected multiannual negative population trends in this species in pristine landscapes of the Southern
Appalachians, whereas its growing populations were found in some of the adjacent areas strongly affected by human
activities. I modified basic Pulliam’s (1988) model of population growth rates for this species by including assumptions
about annual female survival and annual fecundity. I also applied productivity data from 110 active nests to determine an
average successful brood size and nesting success. Finally, I added probabilistic variables accounting for renesting rates
after unsuccessful breeding attempt and double-brooding rates to the model while assuming equal sex ratio among the
breeding individuals. Computer simulations based on actual data and assumed range of values of the model variables yielded
population growth rates well below 1, thus confirming the declining status of the national park populations. Therefore, the
best pristine habitats in the study area were not ecologically significant sources, and in fact they were ecological traps for
this species. Such unpredictable population dynamics in high quality habitats vs. low quality patches could be caused by the
“paradox of predation”: high quality landscapes of the national park attracted, in addition to birds, a variety of mammalian
and reptilian nest predators. Most of these predators were absent or scarce in low quality
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-092-100
Views: 36
About the promotion of natural restoration of Pinus sylvestris L.
N. P. Savinykh, E. V. Lelekova, M. N. Shakleina
Section: Population ecology
The research was carried out in the pine forest of the specially protected natural areas “Medvedsky Bor” in the
Nolinsky District of the Kirov Region. One can meet there both typical boron and boreal plant species and some representatives
of broad-leaved forests and steppes. We researched the composition of vegetation and flora, as well as the
state of Pinus sylvestris L. of the overgrown strip that had been formed after the gradual next-but-one belt logging, with
contribution to renewal (mineralization) and care (clarification-logging with removal of non-target species). Green-moss
and reed vegetation associations prevail there, as it was stated. Vascular plants are analyzed in relation both to humidity
and the spectrum of ecologo-coenotic groups. Predominance of mesophilic and xeromesophilic groups was stated, which
indicates moisture degree sufficient for seed germination of the xeromesophilic P. sylvestris species. Boreal, boron, as
well as non-moral and steppe species prevail, which confirms conservation of biodiversity in these forests. The pine undergrowth
was analyzed as for its number, vitality; vegetation influence on these indicators was stated. It was found out,
per hectare there are 21 thousand young pine plants aged from 4 to16 years. This 84 times exceeds the required number
of trees in the mature community (250 trees per hectare). The vitality of the pine undergrowth is estimated according to
the original method, taking into account the age of the plants, their height, the length of last annual increment and the
mean annual increment, deviation of lateral shoots from the major axis, presence of re-crowning and yellowed leaves. It
is established that there are 17 600 young plants with a high degree of vitality per hectare. It 4.4 times exceeds the plants
number required for artificial restoration of pine forests (4000 trees per hectare). These are the trees which can form pine
forests with a high appraisal index in the future.
Keywords: reforestation, logging, mineralization, undergrowth, real vitality, forest care, biodiversity
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-101-106
Views: 36
The change in soil actinobiote under the influence of Heracleum sosnowskyi invasion
E. V. Tovstik, I. G. Shirokikh, E. S. Solovеva, A. A. Shirokikh, T. Ya. Ashikhmina, V. P. Savinykh
Section: Population ecology
Certain plant species, as well as phytocenosis, can significantly influence the structure and diversity of soil microbiocenosis.
Actinomycetes are an integral component of a soil microbiocenosis that carries out important environmental
functions, inter alia, with the transformation of organic matter. This study was carried out to reveal differences
in the structure of soil actinomycetes complexes between sites overgrown with the Heracleum Sosnowskyi Manden and
non-invasive (control) plots. An increase in genus and species diversity of actinomycetes in invaded soils was noted,
compared with control, in particular, the representatives of the genus Streptosporangium were found exclusively on
hogweed-occupied plots. The appearance of Streptosporangium in the structure of soils actinomycete complexes under
hogweed may indicate that hogweed root exudates contain compounds attractive for this mycelial prokaryotes genus. On
the same plots, an increase in the proportion of colored streptomycetes representing the section and series of Cinereus
Chromogenes was noted, whereas in the control soils streptomycetin complex, the non-producing pigments were dominated
by representatives of the section and series of Cinereus Achromogenes. In the areas of mass growth of Heracleum
Sosnowskyi, in comparison with the control plots, a lower carbon content in the soil was noted, which, in addition to the
changes in the structure of actinomycete complexes, confirms the fact of intensive organic matter mineralization in soils
under the hogweed. Despite the considerable above-ground biomass, at the end of vegetation the removal of nutrients is
not replenished by the hogweed plant litter.
Keywords: Heracleum sosnowskyi Manden., soil, invasion, actinomycetes, total number, species diversity, structure of the complex
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-105-109
Views: 36
Preliminary data about algae and cyanobacteria of volcanic soils on Kuril islands
K. V. Ilchibaeva, D. F. Kunsbaeva, R. Z. Allaguvatova, A. I. Fazlutdinova, O. V. Polokhin, L. A. Sibirina, A. A. Gontcharov, P. Singh, L. A. Gaysina
Section: Population ecology
Fifty-five species from five phyla (Cyanobacteria – 8, Chlorophyta – 29 (Chlorophyceae – 14, Trebouxiophyaceae
– 11), Bacillariophyta – 19, Streptophyta – 1, Ochrophyta – 2 (Xanthophyceae – 1, Eustigmatophyceae – 1)) were revealed
during the study on biodiversity of cyanobacteria and algae from terrestrial habitats of Urup, Paramushir, Iturup,
and Simushir (Kuril Islands). Algal flora of Iturup Islands was the most divers with 25 species, 23 species were found
on Urup, 17 on Simushir, and 14 on Paramushir Islands. Number of species per sample decreased from Urup (3.6) to
Simushir (2.1), Paramushir (2.0) and Iturup (1.9). Five species, cf. Mychonastes homosphaera, Chlorella vulgaris, Dic�
tyococcus varians, Bracteacoccus minor, and Desmodesmus abundans were encountered on each of the island. In the same
time species composition of cyanobacteria and algae on each island was specific and reflected ecological peculiarities of
the habitats. The most notable feature of algal and cyanobacterial flora of studied islands was prevalence of cosmopolitan
species tolerant to toxic volcanic substrates. Besides, this territory was characterized by high diversity of amphibian
diatom algae. For understanding biodiversity of terrestrial algae and cyanobacteria of Kuril islands further moleculargenetic
research are necessary.
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-110-017
Views: 37
The partial replacement of antibiotics with biologically active substances at treatment of cows’ mastitis
M. A. Aziamov
Section: Social ecology
The World Health Organization (WHO) is informing about the antibiotic resistant strains of Staphylococcus aureus
increasing problem. These bacteria are changing their genomic composition under the influence of antibiotics and are rapidly
spreading in the environment, threatening human health and food security in a number of countries. WHO requires reducing
the use of antibiotics in cows’ mastitis by 50%. Staphylococcus aureus is the prevailing kind of cows’ mastitis pathogen in
Western Europe and Russia. The staphylococcal mastitis caused by multiresistant strains of Staphylococcus aureus is registered
in almost 90% of large farms and complexes where antibiotics are used.
The possibility of partial replacement of antibiotics with biologically active substances at treatment of clinical mastitis in
cows was evaluated. It is established that Interferon bovine recombinant, Polysaccharide of Hericium erinaceus fungus (PS of
H. еrinaceus) and Dialderon after 10 days of treatment of clinical mastitis in cows increased the amount of cured individuals,
respectively, by 30, 20 and 20% in comparison with antibiotic therapy. Biologically active substances normalized the number of
somatic cells in milk to physiological level. The studied substances reduced the levels of interleukin-2 (Il-2) and interleukin-8
(Il-8), prostaglandins E2, normalized erythrocyte sedimentation rate (ESR) and phagocytic activity in the blood of experimental
animals. The somatic cells number in the milk of experimental groups cows are decreased to physiological norm after
10 days of treatment. Milk after treatment of cows was fit for food consumption 72 hours earlier than with antibiotic therapy.
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-118-125
Views: 36
Assessment of titanium dioxide nanoparticle effects on living organisms
V. I. Polonskiy, A. A. Asanova
Section: Theoretical problems of ecology
Nanoparticles causing an unprecedented type of industrial pollution directly affect on all objects of the environment and
therefore on all types of living organisms. So, these risks have to be evaluated. This review analyzes the world literature about
effects of titanium dioxide nanoparticles on living organisms of various habitats. Currently, ecotoxicity issues of engineered
nanoparticles are studied using bioassays with cell cultures and test organisms. The most commonly used test objects for
toxicity assessment of nanoparticles are unicellular algae, water crustaceans, plants, mammalian and human cells. It has been
established that the most sensitive organisms to titanium dioxide nanoparticles are unicellular algae and water crustaceans,
EC
50
values were observed at a concentration of 1 mg/L. This suggests that aquatic ecosystem is one of the most vulnerable
objects of the environment to nanoparticles. The high sensitivity of these organisms places them on a par with very promising
biotest assays for quality monitoring of the environment which is contaminated with silver nanoparticles. Titanium dioxide
nanoparticles have both positive and negative or neutral effects upon plants and these effects depend on concentration. The assays
with mammalian and human cells show a negative effect of titanium dioxide nanoparticles at concentration above 100 mg/L
or have no effect at all. The size-depended toxicity analyses revealed that the less nanoparticles size was the greater toxic effect
was. In the final analysis, the responses of living organisms of various habitats to the presence of titanium dioxide nanoparticles
in the environment are insufficiently investigated. There is insufficient data in world literature about size-dependent toxicity
of these nanoparticles to various organisms. These issues require further study.
ISSN 1995-4301
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