Antibiotic sensitivity of Klebsiella pneumoniae and Pseudomonas aeruginosa strains isolated from surface water and wastewater
D.A. Sedova, M.A. Sazykina, P.V. Zhuravlev, I.S. Sazykin, E.A. Egorova, I.S. Berezinskaya, T.I. Tverdokhlebova
Section: Monitoring of natural and anthropogenically disturbed areas
Pseudomonas aeruginosa (Schroeter) Migula) and Klebsiella (Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Ørskov) are opportunistic bacteria with etiological significance in multidrug-resistant infections occurrence. The presence of these bacteria strains in water bodies may indicate anthropogenic fecal contamination. Strains in wastewater and surface waters may represent an antibiotic-resistant bacteria reservoir. The aim of the study was to assess the antibiotic sensitivity of Klebsiella pneumoniae and Pseudomonas aeruginosa strains isolated from the Don and Temernik rivers in the Rostov-on Don and Azov water areas, as well as wastewater from sewage treatment plants in Aksai and Novoshakhtinsk. To recover and enrich the microorganisms, the water samples were inoculated onto both liquid and solid media in accordance with established methodological guidelines, using a serial dilution technique. Bacterial isolates were identified via biochemical tests and mass spectrometric analysis (Microflex LT MALDI-TOF MS). Antibiotic susceptibility testing was performed using the disk diffusion method according to EUCAST clinical recommendations. In total we identified 22 strains of Klebsiella pneumoniae and 76 strains of Pseudomonas aeruginosa. The Klebsiella isolates demonstrated absolute susceptibility to ciprofloxacin, aminoglycosides, chloramphenicol, and meropenem; however, certain resistant phenotypes were observed against amoxicillin-clavulanate, nitrofurantoin, and cefepime. Pseudomonas aeruginosa strains exhibited complete susceptibility to fluoroquinolones and aminoglycosides, though up to 25% of the isolates were resistant to imipenem and meropenem.
Furthermore, several multidrug-resistant strains were detected, displaying simultaneous resistance to multiple β-lactam antibiotics (cefepime, imipenem, and meropenem). The findings of this study confirm that both surface water bodies and wastewater can serve as reservoirs for the dissemination of multidrug-resistant bacteria.
ISSN 1995-4301
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