Prokaryotic test models for environmental ecotoxicological research: prospects and classification
N.V. Dudchik, S. I. Sychik, V. V. Shevlyakov
Section: Theoretical problems of ecology
Biological modeling in system ecology is an effective technology for assessing the biological effects of environmental
factors. We provided the conceptual justification for the development of prokaryotic laboratory-analytical models, assessed
the advantages and limitations of their experimental use. The classification of prokaryotic test models according
to the level of the structural and ecological organization, the properties of the test object (cells, communities, populations,
microbiocenosis) and a set of test reactions (bioindicators and biomarkers) is proposed. The definitions of the test
model as a unified system including a test organism, a biomarker / bioindicator system, a test procedure in accordance
with guidelines of good laboratory practice (GLP), a criteria apparatus for qualitative / quantitative assessment of environmental
factors are given. The concepts of subpopulation and suprapopulation test models based on the properties of
microbial communities as integral biological units regulating vital activity parameters depending on external factors are
substantiated. Have been shown that microorganisms are relevant test objects for the development of innovative methods
for assessing the impact of the physical, chemical and biological environmental factors on organism, which is based on the structural and ecological organization and the variety of metabolic, dynamic, morphological, molecular-genetic
and other properties of microorganisms, integrity microbial culture as a system, cyclical development of populations of
microorganisms in environment as well as their complex interaction with environmental factors.
Keywords: microbiotests, prokaryotic test-models, classification, biomarkers, bioindicators, associations of microorganisms
Article published in number 4 for 2018 DOI: 10.25750/1995-4301-2018-4-005-012
ISSN 1995-4301
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