Comparative evaluation of nutrient media for the cultivation of micromycetes of the genus Trichoderma
P.A. Starikov, L.I. Domracheva, S.G. Skugoreva
Section: Methodology and research methods. Models and forecasts
Fungi of the genus Trichoderma are one of the most used and promising objects of biotechnology. Therefore, among the tasks in the practical application of this micromycete in the future is the isolation of new active strains from the environment and the expansion of the range of culture media used for trichoderma. In this work, we studied the dynamics of vegetative growth of fungi of the genus Trichoderma from various ecological niches. We used isolates from the surface of decaying wood, fruiting bodies of polypores, as well as a strain from urban soil, which was cultivated for 10 years on a medium with sevilen polymer. Cultivation was carried out at 23°С on 5 agar nutrient media, namely, on bean agar, potato and potato-sucrose medium (20 g of sucrose per liter), on the classical Czapek medium, and also on decoction of Fomitopsis pinicola (200 g of fruiting bodies per 1 l of broth). The maximum rates of growth and formation of conidia were observed during surface cultivation of these micromycetes on bean agar and potato-sucrose medium. On the same media of Trichoderma sp., sevilen destructor showed a significant lag in the rate of linear growth (7.55–8.28 mm/day) in comparison with the other studied strains (19.72–24.32 mm/day) which may be explained by profound metabolic changes during long-term cultivation on media where the only source of carbon is a synthetic polymer. The least suitable substrate for cultivation of all studied isolates of Trichoderma spp. turned out to be an agar decoction of polypore, the growth rate on which was 3.55–12.06 mm/day. In all variants of the cultivation of fungi of the genus Trichoderma, the linear growth rate was constant throughout the entire process of cultivation.