Data on leaf damage by pathogenic organisms and the effect of phytopathogens on the content of plastid pigments and the intensity of lipid peroxidation (LPO) processes in the leaves of woody plants are presented. The studies were carried out in the city of Kirov and its environs (southern taiga subzone). The assessment of damage to the leaves of woody plants by pathogens was carried out in different parts of the city in parks and on the territory of the experimental garden of the FGBNU FARC of the North-East. To determine the degree of damage to woody plants, 100 leaves were collected from each plot (10 leaves from 10 trees or shrubs). Leaves were sampled in the first half of the day to determine photosynthetic pigments and the intensity of LPO processes. The content of chlorophylls and carotenoids was determined in acetone extract.
The intensity of lipid peroxidation processes was assessed by the accumulation of malonic dialdehyde (MDA). Photometric measurements were carried out on a ПЭ-5300 ВИ spectrophotometer (Russia). During the study period, 25 species of phytopathogenic fungi were found on 17 species of woody plants, the most common groups of infectious diseases are spotting, rust and powdery mildew are less common. Virus infections (mosaics) make an insignificant contribution to the total number of infectious diseases of woody plants. Diseases caused by fungi and viruses induced similar non-specific responses in the leaves of woody plants, which manifested themselves in a decrease in the level of photosynthetic pigments. In infected leaves, the content of chlorophylls and carotenoids was lower than in healthy leaves, the ratio of pigments in most cases did not change. An increased accumulation of MDA in infected plant cells indicates a progressive destruction of membranes. A change in the color of leaves affected by phytopathogens may be a result of the oxidative degradation of pigment molecules under the action of active radicals formed during LPO. Differences in the intensity of biochemical changes in leaves in response to the action of phytopathogens are determined by the physiological, biochemical and morphological characteristics of different types of woody plants.
ISSN 1995-4301
Select viewing options
