In this study the effect of oxidative stress caused by subchronic intoxication with the fungicide thiram on the antioxidant defense system of the body was examined and the methods for correcting the antioxidant status were developed. As a result of the introduction of thiram, there was a significant increase in the amount of reactive oxygen species (ROS) (~ 3.26 times in relation to the control), together with a significant decrease in reduced glutathione (GSH) (~ 2.63 times) and total antioxidant activity (AAA) (~ 1.64 times) in blood plasma. The level of 8-oxo-2’-deoxyguanosine (8-OHdG) was also significantly higher (p <0.05) in the intoxication groups compared with the control values. The use of vitamin E and milk thistle restored the redox potential of cells. Based on this study it can be assumed that 8-OHdG is a biomarker for assessing the risks associated with excessive production of free radicals.Thiram is a contact fungicide, considered a seed dressing for many agricultural crops. This preparation has high cumulative, toxic properties and can be preserved in agricultural processing products for up to one and a half years. In the present study, we studied the effect of oxidative stress caused by subchronic tiram intoxication on the antioxidant defense system of the rat organism and developed methods for correcting the antioxidant status. Pesticide ingestion resulted in a significant increase in the amount of reactive oxygen species (ROS) (~3.26 times compared to control), a decrease in reduced glutathione (GSH) (~2.63 times) and total antioxidant activity (TAA) (~ 1.64 times) in blood plasma. The level of 8-oxo-2’-deoxyguanosine (8-OHdG) was significantly higher (p<0.05) in the intoxicated groups compared to the control values. After experimental subchronic intoxication, antioxidants were used – vitamin E at a dose of 8.58 mg/kg and milk thistle extract at a dose of 13.74 mg/kg. Their application within 30 days significantly restored the redox potential of the body cells of laboratory animals. The highest antioxidant properties in our study were noted with vitamin E, which allows us to recommend it as a means of correcting the effects caused by the action of oxidative stress in the body. Based on this study, it can be assumed that 8-OHdG is a biomarker for assessing the risks associated with excessive formation of free radicals during oxidative stress. The results of this study can be used in the development of antioxidant therapy to alleviate the effects associated with oxidative stress.
Keywords: thiram, reduced glutathione, 8-oxo-2’-deoxyguanosine, total antioxidant activity, reactive oxygen species (ROS)
Article published in number 2 for 2025 DOI: 10.25750/1995-4301-2025-2-183-189
ISSN 1995-4301
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