The relevance of preserving the genetic resources of the animal and plant world necessitates the search for biologically active substances for the preservation of reproductive cells. Under conditions of hypothermic storage, the osmosis of the extracellular environment, stabilization of membranes and the structure of the cytoskeleton of cells can be provided by various sugars. Xylotrophic Basidiomycete Hericium erinaceus (Bull .: Fr.) Pers. is a source of polysaccharides with high biological activity. Residues of galactose, glucose, arabinose, mannose, fucose, rhamnose, xylose (in order of decreasing % content) were identified in the carbohydrate chains of the polysaccharide fraction (PP) of H. erinaceus BP 16. The effect of PP of different concentrations on the freezing of water in an aqueous solution of glycerin, as well as on the viability of spermatozoa of Holstein bulls under hypothermic (+ 4 °C) storage conditions, on the intensity of LPO processes and antioxidant activity, on the ability of gametes for progressive movement, on sperm resistance to hypoosmotic stress. In connection with the search for new effective components for preserving solutions, the data obtained in this work indicate the prospect of using the PP of H. erinaceus as a component to slow down the rate of ice crystallization in cell suspensions during freezing and as an antioxidant regulator of the functional usefulness of gametes during cooling