Cultivation of Spirulina platensis (Nordst.) Geitler on waste water of poultry farms
S.Yu. Gorbunova, I.N. Gudvilovich
Section: Ecologization of industry
The investigation of cyanobacteria Spirulina (Arthrospira) platensis growth in the chicken manure extract (MCE) was carried out. The content of nitrogen and phosphorus in 1 liter of nutrient medium prepared on the basis of chicken manure extract is calculated. In this case, the protein and photosynthetic pigments contents in the cells of the S. platensis is significantly reduced by the 6th days and leads to the culture death. Increasing the volume fraction of the MCE in the nutrient medium has a significant impact on the pigments contents and total protein in the cyanobacteria biomass. Optimum concentrations of extract (20–30%), which provide a high rate of spirulina growth and the accumulation of biologically active substances in the obtained biomass, were determined. It was shown that 20–30% of MCE concentration allows to increase culture yield by 10–20% compared to control sample and to obtain the S. рlatensis biomass containing significant amounts of biologically active substances. It was found that the content of protein, chlorophyll a and C-phycocyanin in the S. рlatensis cells increased by 2.5–6 times at the linear stage of culture growth at the increase of the volume fraction of MCE in the nutrient medium by 5 to 30%. S. рlatensis biomass, produced from such cultivation process, meets the criteria of high-quality biomass contents of protein, chlorophyll a, carotenoids and C-phycocyanin (51, 1.1, 0.4 and 5.5% correspondingly). The approach proposed in the paper allows to reduce material costs for the traditional mineral media preparation for microalgae cultivation.
Keywords: Spirulina (Arthrospira) platensis, microalgae, chicken manure, biologically active substances
Article published in number 4 for 2020 DOI: 10.25750/1995-4301-2020-4-068-074